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A self-enhanced electrochemiluminescent ratiometric zearalenone immunoassay based on the use of helical carbon nanotubes

Authors
  • Fang, Dandan1
  • Zeng, Baoshan1
  • Zhang, Shupei2
  • Dai, Hong1
  • Lin, Yanyu1, 2
  • 1 Fujian Normal University, Fuzhou, Fujian, 350108, China , Fuzhou (China)
  • 2 Fujian Provincial Maternity and Children’s Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, 350108, China , Fuzhou (China)
Type
Published Article
Journal
Microchimica Acta
Publisher
Springer-Verlag
Publication Date
Apr 30, 2020
Volume
187
Issue
5
Identifiers
DOI: 10.1007/s00604-020-04278-8
Source
Springer Nature
Keywords
License
Yellow

Abstract

A self-enhanced electrochemiluminescent ratiometric immunoassay for zearalenone is described. A system composed of N-aminobutyl-N-ethylisoluminol (ABEI) and glutathione (GSH) produces a strong electrochemiluminescence (ECL) at an applied potential of 0.8 V, probably because of short electron transfer distance and reduced energy loss. The method also uses octahedral anatase mesocrystals (OAM) with a large specific surface facilitating immobilization of ABEI and GSH. Helical carbon nanotubes, possessing a large specific surface, superior mechanical stability, and excellent electrical conductivity which serve as a solid support, greatly enhanced the loading capacity for g-C3N4 nanosheets and horseradish peroxidase-labeled anti-antibody. The peroxidase accelerates the decomposition of H2O2 to produce reactive oxygen species (ROSs), amplifying the blue ECL of ABEI and the green ECL of g-C3N4. The ratiometric sandwich immunoassay (performed by the ratio of ECL intensity at − 1.3 V and 0.8 V) allows for sensitive and reliable determination of ZEN in a wide linear range from 1.0 × 10−4 ng/mL to 10 ng/mL. The method was successfully applied to the analysis of corn hazelnut samples for ZEN. Graphical abstractSchematic presentation of a self-enhanced electrochemiluminescent ratiometric immunosensor based on octahedral anatase mesocrystals (OAM) supported ABEI-glutathione (GSH) and g-C3N4 functionalized helical carbon nanotubes (HCNT) for zearalenone (ZEN) determination.

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