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Selectivity of amino-, cyano- and diol-bonded silica in reversed-phase liquid chromatography.

Authors
  • Kim, I W
  • Lee, H S
  • Lee, Y K
  • Jang, M D
  • Par, J H
Type
Published Article
Journal
Journal of Chromatography A
Publisher
Elsevier
Publication Date
Apr 27, 2001
Volume
915
Issue
1-2
Pages
35–42
Identifiers
PMID: 11358260
Source
Medline
License
Unknown

Abstract

Amino-, cyano- and diol-bonded silica stationary phases were characterized by estimating their characteristic interaction constants in reversed-phase liquid chromatography (RPLC) based on linear solvation energy relationships. Five characteristic interaction constants of the stationary phases, the hydrophobicity (v), polarizability (r), dipolarity (s), hydrogen bond (HB) acceptor basicity (a) and HB donor acidity strength (b) were determined by multiple regression analyses of logarithmic retention factors (k) for a set of test solutes measured on them in 10% (v/v) methanol-water vs. the solute properties represented by characteristic molecular volume (Vx), excess polarization (R2), dipolarity/polarizability (pi*), HB donor acidity (alpha) and HB acceptor basicity (beta). Magnitudes of the five constants for the phases in RPLC were compared with those in normal-phase LC to see the differences in chromatographic selectivity in the two LC modes.

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