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Selective replication of bacteriophage phi29 deoxyribonucleic acid in 6-(p-hydroxyphenylazo)-uracil-treated Bacillus subtilis.

Authors
  • Schachtele, C F
  • Reilly, B E
  • De Sain, C V
  • Whittington, M O
  • Anderson, D L
Type
Published Article
Journal
Journal of virology
Publication Date
Jan 01, 1973
Volume
11
Issue
1
Pages
153–155
Identifiers
PMID: 4630798
Source
Medline
License
Unknown

Abstract

Phage phi29 deoxyribonucleic acid (DNA) replicated under conditions where semiconservative DNA production in Bacillus subtilis host cells was blocked with 6-(p-hydroxyphenylazo)-uracil (HPUra). The time of initiation of phi29 DNA replication was not affected by HPUra, and normal quantities of viable phage were produced in the presence of the inhibitor. Studies with conditional lethal mutants of phage phi29 demonstrated the usefulness of HPUra for detection of viral-specific DNA production.

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