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Selective protection of in vitro synthesized cDNA against nucleases by incorporation of phosphorothioate-analogues.

Authors
  • G Schreiber
  • E M Koch
  • W J Neubert
Publication Date
Nov 11, 1985
Source
PMC
License
Unknown

Abstract

The conditions for the stepwise synthesis of single- (ss) and double-stranded (ds) cDNA using thio-analogues instead of dNTPs are described in this paper. RNA of paramyxovirus Sendai (strain 6/94) serves as template in these experiments. The increased resistance of this alpha S-modified cDNA against several nucleases, like S1-Nuclease, DNase I, Exonuclease III, snake venom Phosphodiesterase (PDE) and the combination of DNase I and PDE is demonstrated.

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