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The selective electrochemical detection of homocysteine in the presence of glutathione, cysteine, and ascorbic acid using carbon electrodes.

Authors
  • Lee, P T1
  • Lowinsohn, D
  • Compton, R G
  • 1 Department of Chemistry, Physical and Theoretical Chemistry Laboratory, Oxford University, South Parks Road, Oxford, OX1 3QZ, UK. [email protected]
Type
Published Article
Journal
The Analyst
Publisher
The Royal Society of Chemistry
Publication Date
Aug 07, 2014
Volume
139
Issue
15
Pages
3755–3762
Identifiers
DOI: 10.1039/c4an00372a
PMID: 24918102
Source
Medline
License
Unknown

Abstract

The detection of homocysteine, HCys, was achieved with the use of catechol via 1,4-Michael addition reaction using carbon electrodes: a glassy carbon electrode and a carbon nanotube modified glassy carbon electrode. The selective detection of homocysteine was investigated and achieved in the absence and presence of glutathione, cysteine and ascorbic acid using cyclic voltammetry and square wave voltammetry. A calibration curve of homocysteine detection was determined and the sensitivity is (0.20 ± 0.02) μA μM(-1) and the limit of detection is 660 nM within the linear range. Lastly, commercially available multi walled carbon nanotube screen printed electrodes were applied to the system for selective homocysteine detection. This work presents a potential practical application towards medical applications as it can be highly beneficial towards quality healthcare management.

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