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A selective and differential agar for anaerobic comma-shaped bacteria recovered from patients having motile rods and non-specific vaginosis.

Authors
  • Thomason, J L
  • Schreckenberger, P C
  • LeBeau, L J
  • Wilcoski, L M
  • Spellacy, W N
Type
Published Article
Journal
Scandinavian journal of urology and nephrology. Supplementum
Publication Date
Jan 01, 1984
Volume
86
Pages
125–128
Identifiers
PMID: 6399404
Source
Medline
License
Unknown

Abstract

A selective and differential agar for optimal growth of the large curved motile anaerobic rods isolated from patients having non-specific vaginosis (NSV) was developed. A basal medium of Columbia CNA agar was used with colony growth found to be optimal by the addition of 7% fetal calf serum and 5% rabbit blood (CNARS). Beta-Haemolysis was found to be demonstrated most readily on a bilayer plate with a basal layer consisting of CNA agar with fetal calf serum, overlaid with a layer of CNA agar containing fetal calf serum and rabbit blood. Optimal growth was found by anaerobic incubation at 37 degrees C, after 5 days.

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