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Selective detection of epimeric pentose saccharides at physiological pH using a fluorescent receptor.

Authors
  • Gibson, Matthew I1
  • Neres, João2
  • Fullam, Elizabeth3
  • 1 Laboratoire des Polymères, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. , (Switzerland)
  • 2 Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. , (Switzerland)
  • 3 Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland. Electronic address: [email protected] , (Switzerland)
Type
Published Article
Journal
Carbohydrate research
Publication Date
Jun 04, 2014
Volume
391
Pages
61–65
Identifiers
DOI: 10.1016/j.carres.2014.02.007
PMID: 24785389
Source
Medline
Keywords
License
Unknown

Abstract

Epimerisation between ribofuranose and arabinofuranose sugars is crucial in several biosynthetic pathways, but is typically challenging to monitor. Here, we have screened for fluorescent boronic acids that can be used as molecular probes for the specific detection of ribofuranose over arabinofuranose sugars in solution. We show excellent specificity of the fluorescent response of 3-biphenylboronic acid to ribofuranose at physiological pH. This provides a tool for in situ monitoring of carbohydrate modifying enzymes and provides a viable alternative to traditional radiolabelled assays.

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