Twenty-six parameters of clinical biochemical properties were determined in 72 clinically healthy German Shepherd dogs. The standard values were determined for total protein, protein spectrum, albuminoglobulin quotient, enzymic activities for AST, ALT, LD, LD-1, and ALP, for sodium, potassium, magnesium, calcium, inorganic phosphorus, and chlorides, complete prameters of the acid-base balance of the blood, and the values of glucose, urea, lactic acid, and creatinin. For determining these standards, dogs were selected at the age of 6.5 months to 7 years. All the statistically processed results are obtained from a number of animals which would secure 95% reliability and accuracy of the results, which would allow for sufficient generalization. Differences concerning the influence of age were not demonstrated in any of the determined biochemical values. The results are regarded as representative standards which can be used for clinical and laboratory diagnostics and prognostics in veterinary cynology and for clinical physiology of the German Shepherd dog breed.