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Screening a library of household substances for inhibitors of phosphatases: An introduction to high-throughput screening.

Authors
  • Taylor, Ann T S
Type
Published Article
Journal
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology
Publication Date
Jan 01, 2005
Volume
33
Issue
1
Pages
16–21
Identifiers
DOI: 10.1002/bmb.2005.494033010430
PMID: 21638534
Source
Medline
License
Unknown

Abstract

Library screening methods are commonly used in industry and research. This article describes an experiment that screens a library of household substances for properties that would make a good "drug," including enzyme inhibition, neutral pH, and nondenaturing to proteins, using wheat germ acid phosphatase as the target protein. An adaptation of the experiment appropriate for lower level biochemistry or outreach is also described. This work was supported by Wabash College through the Haines Fund for the Study of Biochemistry and the National Science Foundation through Grant DUE 0126242.

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