Affordable Access

deepdyve-link
Publisher Website

Screening and Identification of a Specific Binding Peptide to Ovarian Cancer Cells from a Phage-Displayed Peptide Library

Authors
  • Zhao, Shuhui1, 2
  • Li, Chunyan1
  • Gao, Yunge1
  • Qian, Luomeng1
  • Dong, Jian1
  • Zhai, Lianghao1
  • Chen, Biliang1
  • Zhang, Jianfang1
  • 1 Xijing Hospital, The Fourth Military Medical University,
  • 2 Department of Obstetrics and Gynecology, Baiyin Central Hospital, Baiyin, 730913 Gansu China
Type
Published Article
Journal
International Journal of Peptide Research and Therapeutics
Publisher
Springer-Verlag
Publication Date
Apr 03, 2021
Pages
1–9
Identifiers
DOI: 10.1007/s10989-021-10206-y
PMID: 33841057
PMCID: PMC8019349
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Unknown

Abstract

To select specific binding peptides for imaging and detection of human ovarian cancer. The phage 12-mer peptide library was used to select specific phage clones to ovarian cancer cells. After four rounds of biopanning, the binding specificity of randomly selected phage clones to ovarian cancer cells was determined by enzyme-linked immunosorbent assay (ELISA). DNA sequencing and homology analysis were performed on specifically bound phages. The binding ability of the selected peptides to SKOV3 cells was confirmed by fluorescence microscopy and flow cytometry. After four rounds of optimized biological panning, phage recovery was 34-fold higher than that of the first round, and the specific phage clones bound to SKOV3 cells were significantly enriched. A total of 32 positive phage clones were preliminarily identified by ELISA from 54 randomly selected clones, and the positive rate was 59.3%. S36 was identified as the clone with best affinity to SKOV3 cells via fluorescence microscopy and flow cytometry. A representative clone of OSP2, S36 is expected to be an effective probe for diagnosis and treatment of ovarian cancer.

Report this publication

Statistics

Seen <100 times