Overall low transfection efficiencies of ploxP-hCCL20-EGFP into HaCaT cells, regardless of transfection method, may either be due to the high molecular weight of the vector or to the fact that this particular cell line may be inherently difficult to transfect.
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The corresponding record at NLM can be accessed at https://www.ncbi.nlm.nih.gov/pubmed/25672883