Screening of HaCaT clones for CCL20 gene knockout and preliminary exploration of gene-targeting vector transfection approaches in this cell line.
- Authors
- Type
- Published Article
- Journal
- Medical Science Monitor Basic Research
- Publisher
- "International Scientific Information, Inc."
- Publication Date
- Jan 01, 2015
- Volume
- 21
- Pages
- 21–28
- Identifiers
- DOI: 10.12659/MSMBR.893143
- PMID: 25672883
- Source
- Medline
- License
- Unknown
Abstract
Overall low transfection efficiencies of ploxP-hCCL20-EGFP into HaCaT cells, regardless of transfection method, may either be due to the high molecular weight of the vector or to the fact that this particular cell line may be inherently difficult to transfect.
