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Screening of HaCaT clones for CCL20 gene knockout and preliminary exploration of gene-targeting vector transfection approaches in this cell line.

Authors
  • Yong, Wang
  • Peng, Daizhi
  • Wang, Lihua
  • Dong, Zhengxue
  • He, Bing
Type
Published Article
Journal
Medical Science Monitor Basic Research
Publisher
"International Scientific Information, Inc."
Publication Date
Jan 01, 2015
Volume
21
Pages
21–28
Identifiers
DOI: 10.12659/MSMBR.893143
PMID: 25672883
Source
Medline
License
Unknown

Abstract

Overall low transfection efficiencies of ploxP-hCCL20-EGFP into HaCaT cells, regardless of transfection method, may either be due to the high molecular weight of the vector or to the fact that this particular cell line may be inherently difficult to transfect.

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