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Screening of the antimycobacterial activity of novel lipophilic agents by the modified broth based method.

Authors
  • Zandhaghighi, Mehdi1
  • Ghazvini, Kiarash1
  • Meshkat, Zahra1
  • Rezaee, Seyed Abdolrahim1
  • Derakhshan, Mohammad1
  • Soleimanpour, Saman1
  • Hadizadeh, Farzin2
  • 1 Antimicrobial Resistance Research Center & Department of Medical Bacteriology and Virology, Bu-Ali Research Institute & Ghaem University Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. , (Iran)
  • 2 Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. , (Iran)
Type
Published Article
Journal
Journal of clinical tuberculosis and other mycobacterial diseases
Publication Date
May 01, 2016
Volume
3
Pages
1–5
Identifiers
DOI: 10.1016/j.jctube.2016.01.001
PMID: 31723679
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Most of the introduced susceptibility methods of Mycobacterium tuberculosis have some disadvantages for screening. Therefore, the selection of susceptibility assay for evaluating candidate agents must be determined case by case. In this study, we evaluated the validity of a modified broth dilution-based assay in comparison to the gold standard proportional method for microbial sensitivity test of new lipophilic compounds candidate as antitubercular agents. The in-vitro susceptibilities of 114 M. tuberculosis strains were separately tested against isoniazid and two lipophilic antitubercular agents (derivative of dihydropyridines) by employing the standard proportional method and a modified broth dilution-based assay. The results for isoniazid testing showed 100% concordance for sensitivity, specificity and reproducibility. In the case of microbial sensitivity test of lipophilic compounds, comparison of the results obtained from these two methods indicates a significant superiority of the modified method over the standard method. Considering the other advantages of this modified method, we concluded that this modified broth dilution-based assay could be utilized effectively for the susceptibility testing of new lipophilic compounds candidate as antitubercular agents. © 2016 Published by Elsevier Ltd.

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