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Screening of antigen-specific antibody-secreting cells.

Authors
  • Kishi, Hiroyuki
  • Jin, Aishun
  • Ozawa, Tatsuhiko
  • Tajiri, Kazuto
  • Obata, Tsutomu
  • Muraguchi, Atsushi
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 2012
Volume
853
Pages
141–150
Identifiers
DOI: 10.1007/978-1-61779-567-1_11
PMID: 22323145
Source
Medline
License
Unknown

Abstract

Screening of antigen-specific antibody-producing cells is a key step for obtaining antigen-specific monoclonal antibodies. In murine system, hybridoma between B-lymphocytes and myeloma cells is used to screen and produce antigen-specific monoclonal antibodies. In human system, good hybridoma-producing system is not available. Instead, transformation of B-lymphocytes with Epstein-Barr viruses is used to obtain antibody-secreting cell lines. Furthermore, phage-display system using molecular biology is recently used to obtain antigen-specific human monoclonal antibodies. Here, we describe the new method for screening antigen-specific antibody-secreting cells at single-cell levels using microwell-array chips. The system can be applied to screen antigen-specific antibody-secreting cells from any animal species.

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