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Scrambling of bands in gel electrophoresis of DNA.

Authors
  • Lalande, M
  • Noolandi, J
  • Turmel, C
  • Brousseau, R
  • Rousseau, J
  • Slater, G W
Type
Published Article
Journal
Nucleic acids research
Publication Date
Jun 24, 1988
Volume
16
Issue
12
Pages
5427–5437
Identifiers
PMID: 2838816
Source
Medline
License
Unknown

Abstract

Under certain conditions of agarose gel electrophoresis, larger DNA molecules migrate faster than smaller ones. This anomalous mobility of DNA, which can lead to serious errors in the measurement of DNA fragment lengths, is related to near-zero velocity conformations which can trap DNA chains during electrophoresis. Intermittent electric fields can be used to alter the chain conformations so as to restore the monotonic mobility-size relationship which is necessary for a correct interpretation of the gel. These data are in agreement with the results of a computer simulation based on a theoretical model of electrophoresis.

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