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SCAPER, a novel cyclin A-interacting protein that regulates cell cycle progression.

Authors
  • Tsang, William Y
  • Wang, Leyu
  • Chen, Zhihong
  • Sánchez, Irma
  • Dynlacht, Brian David
Type
Published Article
Journal
The Journal of cell biology
Publication Date
Aug 13, 2007
Volume
178
Issue
4
Pages
621–633
Identifiers
PMID: 17698606
Source
Medline
License
Unknown

Abstract

Cyclin A/Cdk2 plays an important role during S and G2/M phases of the eukaryotic cell cycle, but the mechanisms by which it regulates cell cycle events are not fully understood. We have biochemically purified and identified SCAPER, a novel protein that specifically interacts with cyclin A/Cdk2 in vivo. Its expression is cell cycle independent, and it associates with cyclin A/Cdk2 at multiple phases of the cell cycle. SCAPER localizes primarily to the endoplasmic reticulum. Ectopic expression of SCAPER sequesters cyclin A from the nucleus and results specifically in an accumulation of cells in M phase of the cell cycle. RNAi-mediated depletion of SCAPER decreases the cytoplasmic pool of cyclin A and delays the G1/S phase transition upon cell cycle re-entry from quiescence. We propose that SCAPER represents a novel cyclin A/Cdk2 regulatory protein that transiently maintains this kinase in the cytoplasm. SCAPER could play a role in distinguishing S phase- from M phase-specific functions of cyclin A/Cdk2.

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