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Scanningless and continuous 3D bioprinting of human tissues with decellularized extracellular matrix.

Authors
  • Yu, Claire1
  • Ma, Xuanyi2
  • Zhu, Wei1
  • Wang, Pengrui3
  • Miller, Kathleen L1
  • Stupin, Jacob4
  • Koroleva-Maharajh, Anna4
  • Hairabedian, Alexandria4
  • Chen, Shaochen5
  • 1 Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
  • 2 Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
  • 3 Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
  • 4 Chemical Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA.
  • 5 Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA; Chemical Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA, 92093, USA. Electronic address: [email protected]
Type
Published Article
Journal
Biomaterials
Publication Date
Feb 01, 2019
Volume
194
Pages
1–13
Identifiers
DOI: 10.1016/j.biomaterials.2018.12.009
PMID: 30562651
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Decellularized extracellular matrices (dECMs) have demonstrated excellent utility as bioscaffolds in recapitulating the complex biochemical microenvironment, however, their use as bioinks in 3D bioprinting to generate functional biomimetic tissues has been limited by their printability and lack of tunable physical properties. Here, we describe a method to produce photocrosslinkable tissue-specific dECM bioinks for fabricating patient-specific tissues with high control over complex microarchitecture and mechanical properties using a digital light processing (DLP)-based scanningless and continuous 3D bioprinter. We demonstrated that tissue-matched dECM bioinks provided a conducive environment for maintaining high viability and maturation of human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and hepatocytes. Microscale patterning also guided spontaneous cellular reorganization into predesigned striated heart and lobular liver structures through biophysical cues. Our methodology enables a light-based approach to rapidly bioprint dECM bioinks with accurate tissue-scale design to engineer physiologically-relevant functional human tissues for applications in biology, regenerative medicine, and diagnostics. Copyright © 2018 Elsevier Ltd. All rights reserved.

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