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SARS-CoV-2 and the COVID-19 disease: a mini review on diagnostic methods

Authors
  • Oliveira, Beatriz Araujo
  • Oliveira, Lea Campos de
  • Sabino, Ester Cerdeira
  • Okay, Thelma Suely
Publication Date
Jan 01, 2020
Source
Scientific Electronic Library Online - Brazil
Keywords
Language
English
License
Green
External links

Abstract

ABSTRACT Coronavirus disease 2019 (COVID-19) is an infectious disease initially reported in China and currently worldwide dispersed caused by a new coronavirus (SARS-CoV-2 or 2019-nCoV) affecting more than seven million people around the world causing more than 400 thousand deaths (on June 8th, 2020). The diagnosis of COVID-19 is based on the clinical and epidemiological history of the patient. However, the gold standard for COVID-19 diagnosis is the viral detection through the amplification of nucleic acids. Although the quantitative Reverse-Transcription Polymerase Chain Reaction (RT-PCR) has been described as the gold standard for diagnosing COVID-19, there are several difficulties involving its use. Here we comment on RT-PCR and describe alternative tests developed for the diagnosis of COVID-19.

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