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Sample dilution to resolve mistaken identification of haemoglobin D as haemoglobin E using the variant automated system.

Authors
  • Thomas, S
Type
Published Article
Journal
Journal of clinical pathology
Publication Date
Mar 01, 1998
Volume
51
Issue
3
Pages
253–254
Identifiers
PMID: 9659274
Source
Medline
License
Unknown

Abstract

High performance liquid chromatography (HPLC) is increasingly being used to estimate variant haemoglobins. A case of haemoglobin S/D (HbS/D) is presented, which was misdiagnosed as haemoglobin S/E (HbS/E) by HPLC. The patient was a 22 year old woman with sickle cell anaemia. Subsequent haemodilution by blood transfusion clearly elucidated the haemoglobin D peak on HPLC. Sample dilution experiments, using the pretransfusion sample, were done resulting in correct elution of the peak in the D window. Troubleshooting in similar problematic haemoglobin variant peaks seen on HPLC can be done by sample dilution.

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