Affordable Access

deepdyve-link
Publisher Website

Salvia miltiorrhiza Lipophilic Fraction Attenuates Oxidative Stress in Diabetic Nephropathy through Activation of Nuclear Factor Erythroid 2-Related Factor 2.

Authors
  • An, Lin1
  • Zhou, Mei1
  • Marikar, Faiz M M T2
  • Hu, Xue-Wen1
  • Miao, Qiu-Yun1
  • Li, Ping1
  • Chen, Jun1
  • 1 * State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, P. R. China. , (China)
  • 2 † Molecular Biology Unit, Sir John Kotelawala Defence University, Kandawala Estate, Ratmalana, Sri Lanka. , (Sri Lanka)
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2017
Volume
45
Issue
7
Pages
1441–1457
Identifiers
DOI: 10.1142/S0192415X17500781
PMID: 28946766
Source
Medline
Keywords
License
Unknown

Abstract

Diabetic nephropathy (DN) is a common cause of chronic kidney disease and end-stage renal disease, which can be triggered by oxidative stress. In this study, we investigated the renoprotective effect of the ethyl acetate extract of Salvia miltiorrhiza (EASM) on DN and examined the underlying molecular mechanism. We observed that EASM treatment attenuated metabolic abnormalities associated with hyperglycemic conditions in the experimental DN model. In streptozotocin (STZ)-induced mice, EASM treatment reduced albuminuria, improved renal function and alleviated the pathological alterations within the glomerulus. To mimic the hyperglycemic conditions in DN patients, we used high glucose (25[Formula: see text]mmol/L) media to stimulate mouse mesangial cells (MMCs), and EASM inhibited high glucose-induced reactive oxygen species. We also observed that EASM enhanced the expression of nuclear factor erythroid-2-related factor 2 (Nrf2), which mediated the anti-oxidant response, and its downstream gene heme oxygenase-1 (HO-1) and NAD(P)H quinone dehydrogenase 1 (NQO1) with concomitant decrease of expression of kelch-like ECH-associated protein 1 (keap1) both in vitro and in vivo. Taken together, these results suggest that EASM alleviates the progression of DN and this might be associated with activation of Nrf2.

Report this publication

Statistics

Seen <100 times