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Saccharomyces cerevisiae and Torulaspora delbrueckii Intra- and Extra-Cellular Aromatic Amino Acids Metabolism.

Authors
  • Álvarez-Fernández, M Antonia1
  • Fernández-Cruz, Edwin1
  • Garcia-Parrilla, M Carmen1
  • Troncoso, Ana M1
  • Mattivi, Fulvio2, 3
  • Vrhovsek, Urska2
  • Arapitsas, Panagiotis2
  • 1 Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia , Universidad de Sevilla , C/P. García González no. 2 , Sevilla 41012 , Spain. , (Spain)
  • 2 Department of Food Quality and Nutrition, Research and Innovation Centre , Fondazione Edmund Mach-Istituto Agrario San Michele all'Adige , Trento , Italy. , (Italy)
  • 3 Center Agriculture Food Environment , University of Trento , Trento , Italy. , (Italy)
Type
Published Article
Journal
Journal of Agricultural and Food Chemistry
Publisher
American Chemical Society
Publication Date
Jul 17, 2019
Volume
67
Issue
28
Pages
7942–7953
Identifiers
DOI: 10.1021/acs.jafc.9b01844
PMID: 31264861
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Tryptophan, phenylalanine, and tyrosine play an important role as nitrogen sources in yeast metabolism. They regulate biomass production and fermentation rate, and their catabolites contribute to wine health benefits and sensorial character through the yeast biotransformation of grape juice constitutes into biologically active and flavor-impacting components. A UHPLC-MS/MS method was applied to monitor 37 tryptophan/phenylalanine/tyrosine yeast metabolites both in extra- and intracellular extracts produced by the fermentation of two Saccharomyces cerevisiae strains and one Torulaspora delbrueckii. The results shed light on the intra- and extra-cellular metabolomic dynamics, by combining metabolic needs, stimuli, and signals. Among others, the results indicated (a) the production of 2-aminoacetophenone by yeasts, mainly by the two Saccharomyces cerevisiae; (b) the deactivation and/or detoxification of tryptophol via sulfonation reaction; and (c) the deacetylation of N-acetyl tryptophan ethyl ester and N-acetyl tyrosine ethyl ester by producing the corresponding ethyl esters.

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