Affordable Access

deepdyve-link
Publisher Website

S1 nuclease protection mapping.

Authors
  • Smith, D R
Type
Published Article
Journal
Methods in Molecular Biology
Publication Date
Jan 01, 1993
Volume
18
Pages
363–372
Identifiers
DOI: 10.1385/0-89603-245-0:363
PMID: 21390683
Source
Medline
License
Unknown

Abstract

The use of S1 nuclease to map the start site of a transcription unit is a well-established technique. Based on the method of Berk and Sharp, it has undergone many refinements over the years. S1 nuclease mapping requires a relatively detailed knowledge of the gene structure and sequence data (or a very good restriction map) of the first exon and several hundred bases of upstream sequence. Although S1 nuclease mapping is mainly used to map transcription start sites accurately (to be described in this chapter), this method can also be used to map intron-exon junctions. Note that in order to identify a transcriptional start site unambiguously, S1 nuclease mapping should be used in conjunction with primer extension (Chapter 44).

Report this publication

Statistics

Seen <100 times