Affordable Access

Ryanodine as a functional probe of the skeletal muscle sarcoplasmic reticulum Ca2+ release channel.

Authors
Type
Published Article
Journal
Molecular and cellular biochemistry
Publication Date
Volume
114
Issue
1-2
Pages
119–123
Identifiers
PMID: 1334225
Source
Medline
License
Unknown

Abstract

Ryanodine is a neutral plant alkaloid which functions as a probe for an intracellular Ca2+ release channel (ryanodine receptor) in excitable tissues. Using [3H]ryanodine, a 30 S protein complex comprised of four polypeptides of Mr 565,000 has been isolated and functionally reconstituted into planar lipid bilayers. The effects of salt concentration and divalent cations on skeletal muscle sarcoplasmic reticulum [3H]ryanodine binding and Ca2+ release channel activity have been compared. These studies suggest that ryanodine is a good probe for investigating the function of the release channel.

Statistics

Seen <100 times