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Rutin and quercetin enhance glucose uptake in L6 myotubes under oxidative stress induced by tertiary butyl hydrogen peroxide

Authors
  • Dhanya, R.
  • Arun, K.B.
  • Syama, H.P.
  • Nisha, P.
  • Sundaresan, A.
  • Santhosh Kumar, T.R.
  • Jayamurthy, P.1, 2, 3, 4
  • 1 Agroprocessing and Natural Products Division
  • 2 National Institute for Interdisciplinary Science and Technology (NIIST)
  • 3 CSIR
  • 4 Rajiv Gandhi Centre for Biotechnology
Type
Published Article
Journal
Food Chemistry
Publisher
Elsevier
Publication Date
Jan 01, 2014
Accepted Date
Feb 26, 2014
Volume
158
Pages
546–554
Identifiers
DOI: 10.1016/j.foodchem.2014.02.151
Source
Elsevier
Keywords
License
Unknown

Abstract

Scientific evidence suggests a strong link between the oxidative stress-induced pathways and onset of diabetes and its complications. The present study evaluates the antidiabetic potential of the flavonoids, rutin and its metabolite quercetin under oxidative stress induced by tertiary butyl hydrogen peroxide (TBHP). Our results demonstrate that reactive oxygen species generated by TBHP decreased markedly in the L6 cells on preincubation with flavonoids in a dose-dependent manner and remarkably retrieved the glutathione level which was drastically decreased on oxidative challenge. These flavonoids were also found to prevent lipid peroxidation in L6 myoblast. Flavonoids increased glucose following chronic and acute pretreatment in the presence of oxidative stress. Increased glucose uptake in L6 myotubes was attributed to GLUT 4 translocation, the most downstream factor in the insulin signalling cascade, which increased two to threefold on chronic pretreatment of quercetin (10μM) and rutin (100μM).

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