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RUNX1 suppression induces megakaryocytic differentiation of UT-7/GM cells

Authors
  • Nagai, Ryohei
  • Matsuura, Eri
  • Hoshika, Yusuke
  • Nakata, Emi
  • Nagura, Hironori
  • Watanabe, Ayako
  • Komatsu, Norio
  • Okada, Yoshiaki
  • Doi, Takefumi
Type
Published Article
Journal
Biochemical and Biophysical Research Communications
Publication Date
Jan 01, 2006
Volume
345
Issue
1
Pages
78–84
Identifiers
DOI: 10.1016/j.bbrc.2006.04.057
Source
Elsevier
Keywords
License
Unknown

Abstract

The transcription factor RUNX1 plays a crucial role in hematopoiesis. RUNX1 regulates both differentiation and proliferation of hematopoietic cells. Several reports have shown that RUNX1 participates in megakaryopoiesis, which is a process that leads to formation of platelets. However, to date, the mechanisms by which this occurs have not been fully elucidated. In the present study, we investigated whether siRNA-mediated depletion of RUNX1 affected megakaryopoiesis of UT-7/GM cells. The depletion of RUNX1 in UT-7/GM cells resulted in up-regulation of the expression of megakaryocytic markers and polyploidization, while cell proliferation was down-regulated. Furthermore, the overexpression of RUNX1 decreased the activity of megakaryocytic gene promoters. These results suggest that RUNX1 down-regulates terminal differentiation of megakaryocytes and promotes proliferation of megakaryocytic progenitors.

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