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Rubus idaeus Inhibits Migration and Invasion of Human Nasopharyngeal Carcinoma Cells by Suppression of MMP-2 through Modulation of the ERK1/2 Pathway.

Authors
  • Hsin, Chung-Han1, 2
  • Huang, Cheng-Chen1, 2
  • Chen, Pei-Ni3, 4
  • Hsieh, Yih-Shou3, 4
  • Yang, Shun-Fa3, 5
  • Ho, Yu-Ting5
  • Lin, Chiao-Wen6, 7
  • 1 * Department of Otolaryngology, Chung Shan Medical University Hospital, Taichung, Taiwan. , (Taiwan)
  • 2 § School of Medicine, Chung Shan Medical University, Taichung, Taiwan. , (Taiwan)
  • 3 † Department of Medical Research, Chung Shan Medical University Hospital, Taichung, Taiwan. , (Taiwan)
  • 4 ¶ Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan. , (Taiwan)
  • 5 ∥ Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan. , (Taiwan)
  • 6 ‡ Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan. , (Taiwan)
  • 7 ** Institute of Oral Sciences, Chung Shan Medical University, Taichung, Taiwan. , (Taiwan)
Type
Published Article
Journal
The American journal of Chinese medicine
Publication Date
Jan 01, 2017
Volume
45
Issue
7
Pages
1557–1572
Identifiers
DOI: 10.1142/S0192415X17500847
PMID: 28946771
Source
Medline
Keywords
License
Unknown

Abstract

Nasopharyngeal carcinoma (NPC) is characterized by a high incidence of metastasis in the neck lymph nodes, resulting in a poor prognosis and posing challenges for treatment. In this study, we investigated the in vitro antimetastatic properties of Rubus idaeus extract (RIE) on human nasopharyngeal carcinoma cells. HONE-1, NPC-39 and NPC-BM cells were subjected to RIE treatment, and effects on the migration and invasion of tumor cells were analyzed. The results showed that RIE suppressed the migration and invasion of NPC cells. Gelatin zymography assay, Western blotting and real-time PCR showed that matrix metalloproteinases-2 (MMP-2) enzyme activity, protein expression and mRNA levels were down-regulated by RIE treatment. To identify the signaling pathway, mitogen-activated protein kinase proteins were examined, which showed that phosphorylation of ERK1/2 was inhibited after the treatment of RIE. In summary, our data showed that RIE inhibited the migration and invasion of NPC cells by suppressing the expression of MMP-2 by down-regulating the ERK1/2 signaling pathway, suggesting that Rubus idaeus may serve as chemotherapeutic and chemopreventive agent for NPC.

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