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Rosiglitazone protects endothelial cells from irradiation-induced mitochondrial dysfunction

Authors
  • Baselet, Bjorn
  • Driesen, Ronald B.
  • Coninx, Emma
  • Belmans, Niels
  • Sieprath, Tom
  • Lambrichts, Ivo
  • De Vos, Winnok
  • Baatout, Sarah
  • Sonveaux, Pierre
  • Aerts, An
Publication Date
Jan 01, 2020
Identifiers
DOI: 10.3389/fphar.2020.00268
OAI: oai:archive.ugent.be:8669233
Source
Ghent University Institutional Archive
Keywords
Language
English
License
Green
External links

Abstract

Background and Purpose: Up to 50–60% of all cancer patients receive radiotherapy as part of their treatment strategy. However, the mechanisms accounting for increased vascular risks after irradiation are not completely understood. Mitochondrial dysfunction has been identified as a potential cause of radiation-induced atherosclerosis. Materials and Methods: Assays for apoptosis, cellular metabolism, mitochondrial DNA content, functionality and morphology were used to compare the response of endothelial cells to a single 2 Gy dose of X-rays under basal conditions or after pharmacological treatments that either reduced (EtBr) or increased (rosiglitazone) mitochondrial content. Results: Exposure to ionizing radiation caused a persistent reduction in mitochondrial content of endothelial cells. Pharmacological reduction of mitochondrial DNA content rendered endothelial cells more vulnerable to radiation-induced apoptosis, whereas rosiglitazone treatment increased oxidative metabolism and redox state and decreased the levels of apoptosis after irradiation. Conclusion: Pre-existing mitochondrial damage sensitizes endothelial cells to ionizing radiation-induced mitochondrial dysfunction. Rosiglitazone protects endothelial cells from the detrimental effects of radiation exposure on mitochondrial metabolism and oxidative stress. Thus, our findings indicate that rosiglitazone may have potential value as prophylactic for radiation-induced atherosclerosis.

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