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The Role of Toll-Like Receptors in the Production of Cytokines by Human Lung Macrophages

Authors
  • Grassin-Delyle, Stanislas
  • Abrial, Charlotte
  • Salvator, Hélène
  • Brollo, Marion
  • Naline, Emmanuel
  • Devillier, Philippe
Type
Published Article
Journal
Journal of Innate Immunity
Publisher
S. Karger AG
Publication Date
Dec 17, 2018
Volume
12
Issue
1
Pages
63–73
Identifiers
DOI: 10.1159/000494463
PMID: 30557876
Source
Karger
Keywords
License
Green
External links

Abstract

Background: The Toll-like receptor (TLR) family is involved in the recognition of and response to microbial infections. These receptors are expressed in leukocytes. TLR stimulation induces the production of proinflammatory cytokines and chemokines. Given that human lung macrophages (LMs) constitute the first line of defense against inhaled pathogens, the objective of this study was to investigate the expression and function of TLR subtypes in this cell population. Methods: Human primary LMs were obtained from patients undergoing surgical resection. The RNA and protein expression levels of TLRs, chemokines, and cytokines were assessed after incubation with subtype-selective agonists. Results: In human LMs, the TLR expression level varied from one subtype to another. Stimulation with subtype-selective agonists induced an intense, concentration- and time-dependent increase in the production of chemokines and cytokines. TLR4 stimulation induced the strongest effect, whereas TLR9 stimulation induced a much weaker response. Conclusions: The stimulation of TLRs in human LMs induces intense cytokine and chemokine production, a characteristic of the proinflammatory M1 macrophage phenotype.

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