Affordable Access

deepdyve-link
Publisher Website

Role of Reelin in cell positioning in the cerebellum and the cerebellum-like structure in zebrafish.

Authors
  • Nimura, Takayuki1
  • Itoh, Tsubasa1
  • Hagio, Hanako2
  • Hayashi, Takuto1
  • Di Donato, Vincenzo3
  • Takeuchi, Miki4
  • Itoh, Takeaki5
  • Inoguchi, Fuduki5
  • Sato, Yoshikatsu6
  • Yamamoto, Naoyuki7
  • Katsuyama, Yu5
  • Del Bene, Filippo3
  • Shimizu, Takashi8
  • Hibi, Masahiko9
  • 1 Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8602, Japan. , (Japan)
  • 2 Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8602, Japan; Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan. , (Japan)
  • 3 Institut Curie, PSL Research University, INSERM U934, CNRS UMR3215, UPMC Paris-Sorbonne, Paris, 75005, France. , (France)
  • 4 Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8601, Japan. , (Japan)
  • 5 Department of Anatomy, Shiga University of Medical Science, Otsu, Shiga, 520-2192, Japan. , (Japan)
  • 6 Institute of Transformative Bio-Molecules, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8601, Japan. , (Japan)
  • 7 Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan. , (Japan)
  • 8 Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8602, Japan; Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8601, Japan. , (Japan)
  • 9 Division of Biological Science, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8602, Japan; Bioscience and Biotechnology Center, Nagoya University, Furo, Chikusa, Nagoya, Aichi, 464-8601, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Developmental Biology
Publisher
Elsevier
Publication Date
Nov 15, 2019
Volume
455
Issue
2
Pages
393–408
Identifiers
DOI: 10.1016/j.ydbio.2019.07.010
PMID: 31323192
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

The cerebellum and the cerebellum-like structure in the mesencephalic tectum in zebrafish contain multiple cell types, including principal cells (i.e., Purkinje cells and type I neurons) and granule cells, that form neural circuits in which the principal cells receive and integrate inputs from granule cells and other neurons. It is largely unknown how these cells are positioned and how neural circuits form. While Reelin signaling is known to play an important role in cell positioning in the mammalian brain, its role in the formation of other vertebrate brains remains elusive. Here we found that zebrafish with mutations in Reelin or in the Reelin-signaling molecules Vldlr or Dab1a exhibited ectopic Purkinje cells, eurydendroid cells (projection neurons), and Bergmann glial cells in the cerebellum, and ectopic type I neurons in the tectum. The ectopic Purkinje cells and type I neurons received aberrant afferent fibers in these mutants. In wild-type zebrafish, reelin transcripts were detected in the internal granule cell layer, while Reelin protein was localized to the superficial layer of the cerebellum and the tectum. Laser ablation of the granule cell axons perturbed the localization of Reelin, and the mutation of both kif5aa and kif5ba, which encode major kinesin I components in the granule cells, disrupted the elongation of granule cell axons and the Reelin distribution. Our findings suggest that in zebrafish, (1) Reelin is transported from the granule cell soma to the superficial layer by axonal transport; (2) Reelin controls the migration of neurons and glial cells from the ventricular zone; and (3) Purkinje cells and type I neurons attract afferent axons during the formation of the cerebellum and the cerebellum-like structure. Copyright © 2019 Elsevier Inc. All rights reserved.

Report this publication

Statistics

Seen <100 times