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Role of the Pre-neck Appendage Protein (Dpo7) from Phage vB_SepiS-phiIPLA7 as an Anti-biofilm Agent in Staphylococcal Species

Authors
  • Gutiérrez, Diana1
  • Briers, Yves2, 3
  • Rodríguez-Rubio, Lorena1, 2
  • Martínez, Beatriz1
  • Rodríguez, Ana1
  • Lavigne, Rob2
  • García, Pilar1
  • 1 Consejo Superior de Investigaciones Científicas – Instituto de Productos Lácteos de Asturias, Villaviciosa, Spain
  • 2 Laboratory of Gene Technology, KU Leuven, Heverlee, Belgium
  • 3 Laboratory of Applied Biotechnology, Ghent University, Ghent, Belgium
Type
Published Article
Journal
Frontiers in Microbiology
Publisher
Frontiers Media SA
Publication Date
Nov 25, 2015
Volume
6
Identifiers
DOI: 10.3389/fmicb.2015.01315
Source
Frontiers
Keywords
Disciplines
  • Microbiology
  • Original Research
License
Green

Abstract

Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-phiIPLA7, was overexpressed in Escherichia coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (>90%) of biofilm-attached cells was obtained with 0.15 μM of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pre-treatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53–85% in the 67% of the tested strains. This study supports the use of phage-encoded EPS depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.

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