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Role of the pre-neck appendage protein (Dpo7) from phage vB_SepiS-philPLA7 as an anti-biofilm agent in Staphylococcal species

  • Gutiérrez, Diana
  • Briers, Yves
  • Rodríguez-Rubio, Lorena
  • Martínez, Beatriz
  • Rodríguez, Ana
  • Lavigne, Rob
  • García, Pilar
Publication Date
Jan 01, 2015
Ghent University Institutional Archive
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Staphylococcus epidermidis and Staphylococcus aureus are important causative agents of hospital-acquired infections and bacteremia, likely due to their ability to form biofilms. The production of a dense exopolysaccharide (EPS) matrix enclosing the cells slows the penetration of antibiotic down, resulting in therapy failure. The EPS depolymerase (Dpo7) derived from bacteriophage vB_SepiS-philPLA7, was overexpressed in Escherichia coli and characterized. A dose dependent but time independent response was observed after treatment of staphylococcal 24 h-biofilms with Dpo7. Maximum removal (>90%) of biofilm-attached cells was obtained with 0.15 mu M of Dpo7 in all polysaccharide producer strains but Dpo7 failed to eliminate polysaccharide-independent biofilm formed by S. aureus V329. Moreover, the pretreatment of polystyrene surfaces with Dpo7 reduced the biofilm biomass by 53-85% in the 67% of the tested strains. This study supports the use of phage-encoded EPS depolymerases to prevent and disperse staphylococcal biofilms, thereby making bacteria more susceptible to the action of antimicrobials.

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