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The Role of Orthogonality in Genetic Code Expansion

Authors
  • arranz-gibert, pol
  • patel, jaymin r.
  • isaacs, farren j.
Publication Date
Jul 05, 2019
Source
MDPI
Keywords
Language
English
License
Green
External links

Abstract

the engineered machinery used to introduce nsAAs into proteins must co-exist with native translation and gene expression without cross-reactivity or pleiotropy. The issue of orthogonality manifests at several levels, including those of codons, ribosomes, aminoacyl-tRNA synthetases, tRNAs, and elongation factors. In this concept paper, we describe advances in genome recoding, translational engineering and associated challenges rooted in establishing orthogonality needed to expand the genetic code.

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