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Role of endocannabinoid CB1 receptors in Streptozotocin-induced uninephrectomised Wistar rats in diabetic nephropathy

Authors
  • Medapati, Jayarami Reddy1
  • Rapaka, Deepthi1
  • Bitra, Veera Raghavulu1
  • Ranajit, Santhosh Kumar2
  • Guntuku, Girija Sankar1
  • Akula, Annapurna1
  • 1 Andhra University, Visakhapatnam, Andhra Pradesh, 530003, India , Visakhapatnam (India)
  • 2 Vignan Institute of Pharmacuetical Technology, Duvvada, Visakhapatnam, India , Visakhapatnam (India)
Type
Published Article
Journal
Beni-Suef University Journal of Basic and Applied Sciences
Publisher
Springer Berlin Heidelberg
Publication Date
May 26, 2021
Volume
10
Issue
1
Identifiers
DOI: 10.1186/s43088-021-00121-y
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundThe endocannabinoid CB1 receptor is known to have protective effects in kidney disease. The aim of the present study is to evaluate the potential agonistic and antagonistic actions and to determine the renoprotective potential of CB1 receptors in diabetic nephropathy. The present work investigates the possible role of CB1 receptors in the pathogenesis of diabetes-induced nephropathy. Streptozotocin (STZ) (55 mg/kg, i.p., once) is administered to uninephrectomised rats for induction of experimental diabetes mellitus. The CB1 agonist (oleamide) and CB1 antagonist (AM6545) treatment were initiated in diabetic rats after 1 week of STZ administration and were given for 24 weeks.ResultsThe progress in diabetic nephropathy is estimated biochemically by measuring serum creatinine (1.28±0.03) (p < 0.005), blood urea nitrogen (67.6± 2.10) (p < 0.001), urinary microprotein (74.62± 3.47) (p < 0.005) and urinary albuminuria (28.31±1.17) (p < 0.0001). Renal inflammation was assessed by estimating serum levels of tumor necrosis factor alpha (75.69±1.51) (p < 0.001) and transforming growth factor beta (8.73±0.31) (p < 0.001). Renal morphological changes were assessed by estimating renal hypertrophy (7.38± 0.26) (p < 0.005) and renal collagen content (10.42± 0.48) (p < 0.001).ConclusionsFrom the above findings, it can be said that diabetes-induced nephropathy may be associated with overexpression of CB1 receptors and blockade of CB1 receptors might be beneficial in ameliorating the diabetes-induced nephropathy.Graphical abstract

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