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Role of Ancillary Techniques in Biliary Cytopathology Specimens

Authors
  • Layfield, Lester
Type
Published Article
Journal
Acta Cytologica
Publisher
S. Karger AG
Publication Date
May 23, 2019
Volume
64
Issue
1-2
Pages
175–181
Identifiers
DOI: 10.1159/000498976
PMID: 31121596
Source
Karger
Keywords
License
Green
External links

Abstract

Biliary brushing cytology has become the standard of practice for the investigation of strictures of the biliary and pancreatic duct systems. The methodology however has a limitation in that it has low diagnostic sensitivity when only cytologic evaluation is used. A number of testing methodologies have been applied to brushing specimens in an attempt to improve overall sensitivity without loss of specificity. These have included DNA ploidy analysis, immunocytochemistry, individual gene mutational analysis, fluorescence in-situ hybridization (FISH), and next generation sequencing (NGS). Currently, FISH coupled with routine cytology appears to be the method of choice for improving diagnostic sensitivity. NGS shows significant promise for improvement of diagnostic sensitivity.

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