Affordable Access

Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer

Authors
  • Nolivos, Sophie
  • Cayron, Julien
  • Dedieu, Annick
  • Page, Adeline
  • Delolme, Frédéric
  • Lesterlin, Christian
Publication Date
May 23, 2019
Source
HAL-INRIA
Keywords
Language
English
License
Unknown
External links

Abstract

Drug-resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the Escherichia coli fertility factor–conjugation plasmid encoding the tetracycline-efflux pump TetA. The entry of the single-stranded DNA plasmid into the recipient cell is rapidly followed by complementary-strand synthesis, plasmid-gene expression, and production of TetA. In the presence of translation-inhibiting antibiotics, resistance acquisition depends on the AcrAB-TolC multidrug efflux pump, because it reduces tetracycline concentrations in the cell. Protein synthesis can thus persist and TetA expression can be initiated immediately after plasmid acquisition. AcrAB-TolC efflux activity can also preserve resistance acquisition by plasmid transfer in the presence of antibiotics with other modes of action

Report this publication

Statistics

Seen <100 times