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RNase H mediated cleavage of RNA by cyclohexene nucleic acid (CeNA).

Authors
  • Verbeure, B
  • Lescrinier, E
  • Wang, J
  • Herdewijn, P
Type
Published Article
Journal
Nucleic Acids Research
Publisher
Oxford University Press
Publication Date
Dec 15, 2001
Volume
29
Issue
24
Pages
4941–4947
Identifiers
PMID: 11812823
Source
Medline
License
Unknown

Abstract

Cyclohexene nucleic acid (CeNA) forms a duplex with RNA that is more stable than a DNA-RNA duplex (DeltaTm per modification: +2 degrees C). A cyclohexenyl A nucleotide adopts a 3'-endo conformation when introduced in dsDNA. The neighbouring deoxynucleotide adopts an O4'-endo conformation. The CeNA:RNA duplex is cleaved by RNase H. The Vmax and Km of the cleavage reaction for CeNA:RNA and DNA:RNA is in the same range, although the kcat value is about 600 times lower in the case of CeNA:RNA.

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