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An RNAi-independent role of AP1-like stress response factor Pap1 in centromere and mating-type silencing in Schizosaccaromyces pombe

Authors
  • Kumar, Ashok1, 2
  • Nanda, Jagpreet Singh1, 3
  • Saini, Sharanjot1, 4
  • Singh, Jagmohan1, 5
  • 1 Institute of Microbial Technology, Sector 39A, Chandigarh, 160 036, India , Chandigarh (India)
  • 2 Agriculture University Kota, Jhalrapatan, 326 023, India , Jhalrapatan (India)
  • 3 Cedars Sinai, Department of Hematology and Oncology, 8700 Beverly Boulevard, Los Angeles, CA, 90048, USA , Los Angeles (United States)
  • 4 Augusta University, 1410 Laney Walker Blvd, Augusta, GA, 30912, USA , Augusta (United States)
  • 5 Panjab University, Chandigarh, 160 014, India , Chandigarh (India)
Type
Published Article
Journal
Journal of Biosciences
Publisher
Springer-Verlag
Publication Date
Jul 30, 2021
Volume
46
Issue
3
Identifiers
DOI: 10.1007/s12038-021-00199-7
Source
Springer Nature
Keywords
Disciplines
  • Article
License
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Abstract

Gene silencing in S. pombe occurs by heterochromatin formation at the centromere (cen), mating-type (mat) and telomere loci. It is mediated by silencing factors including Swi6, Clr1-4, Rhp6 and Polα. RNAi pathway also plays a role in establishment of silencing at the mat and cen loci. Recently, the stress response factors, Atf1 and Pcr1were shown to play an RNAi-independent role in silencing at the mat3 locus through a cis-acting Atf1-binding site located within the repression element REIII and recruitment of the silencing factors Clr3 and Clr6. Another cis-acting site, named repression element REII abutting the mat2 locus, also establishes heterochromatin structure through Clr5 and histone deacetylases but independently of H3-Lys9-methylation and RNAi. Here, we report the occurrence of binding sites for another oxidative response factor, the pombe AP1-like factor Pap1, at the mating-type, centromere and telomere loci. By genetic studies we show that these sites play a role in silencing at the outer repeats of centromeres as well as mating-type locus and this effect is mediated through Pap1 binding site and interaction with and recruitment of the HP1/Swi6. Importantly, pap1Δ cells display a silencing defect even in absence of the oxidative stress. Such a role of Pap1 in heterochromatin formation may be evolutionarily conserved.

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