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RNA viruses in the house dust mite Dermatophagoides pteronyssinus, detection in environmental samples and in commercial allergen extracts used for in vivo diagnosis.

Authors
  • Vidal-Quist, José Cristian1
  • Vidal, Carmen2
  • Escolar, Fernando3
  • Lambrecht, Bart N4, 5
  • Rombauts, Stephane6, 7
  • Hernández-Crespo, Pedro1
  • 1 Laboratorio de Interacción Planta-Insecto, Departamento de Biotecnología Microbiana y de Plantas, Centro de Investigaciones Biológicas Margarita Salas - CSIC, Madrid, Spain. , (Spain)
  • 2 Servicio de Alergología, Complejo Hospitalario Universitario de Santiago (CHUS), Santiago de Compostela, Spain. , (Spain)
  • 3 Servicio de Microscopía Electrónica, Centro de Investigaciones Biológicas Margarita Salas - CSIC, Madrid, Spain. , (Spain)
  • 4 Laboratory of Immunoregulation and Mucosal Immunology, VIB Center for Inflammation Research, Ghent, Belgium. , (Belgium)
  • 5 Department of Internal Medicine and Pediatrics, Ghent University, Ghent, Belgium. , (Belgium)
  • 6 Center for Plant Systems Biology, VIB, Ghent, Belgium. , (Belgium)
  • 7 Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium. , (Belgium)
Type
Published Article
Journal
Allergy
Publication Date
Dec 01, 2021
Volume
76
Issue
12
Pages
3743–3754
Identifiers
DOI: 10.1111/all.14884
PMID: 33914957
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Allergy to house dust mites (HDM), the most important source of indoor allergens worldwide, is diagnosed and treated using natural extracts from cultures that can contain immunoactive components from the HDM microbiome, including mite-infecting viruses. This study aimed to contribute to the discovery and characterization of RNA viruses from Dermatophagoides pteronyssinus, followed by their detection in different mite-derived sources. Viruses were assembled after in silico metatranscriptomic analysis of D. pteronyssinus RNA samples, visualized by electron microscopy, and RNA detected by direct RT-PCR or data mining. Mite culture performance was evaluated in vivo. Seven RNA viruses were identified in our laboratory stock colony. Picornavirus-like viral particles were detected in epithelial cells of the digestive system and in fecal pellets. Most of these viruses could be persistently transmitted to an inbred virus-free colony by inoculating fecal material from the stock colony. Upon viral infection, no significant effect could be seen on mite population growth. Transcriptomic screening confirmed the presence of homolog sequences to these viruses in independent laboratory stocks of D. pteronyssinus and in other Astigmata mites. Noteworthy, RNA from most of the viruses could be detected by RT-PCR on house dust samples, reference standards, and/or commercial diagnostic D. pteronyssinus extracts. Our results show that viral infections are common and widespread in D. pteronyssinus, both in natural and culture-based growth conditions. Potential effects on the mites themselves and consequences toward allergenicity in humans whether exposed naturally or after immunotherapy are discussed. © 2021 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.

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