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An RNA motif that binds ATP.

Authors
  • Sassanfar, M
  • Szostak, J W
Type
Published Article
Journal
Nature
Publication Date
Aug 05, 1993
Volume
364
Issue
6437
Pages
550–553
Identifiers
PMID: 7687750
Source
Medline
Keywords
License
Unknown

Abstract

RNAs that contain specific high-affinity binding sites for small molecule ligands immobilized on a solid support are present at a frequency of roughly one in 10(10)-10(11) in pools of random sequence RNA molecules. Here we describe a new in vitro selection procedure designed to ensure the isolation of RNAs that bind the ligand of interest in solution as well as on a solid support. We have used this method to isolate a remarkably small RNA motif that binds ATP, a substrate in numerous biological reactions and the universal biological high-energy intermediate. The selected ATP-binding RNAs contain a consensus sequence, embedded in a common secondary structure. The binding properties of ATP analogues and modified RNAs show that the binding interaction is characterized by a large number of close contacts between the ATP and RNA, and by a change in the conformation of the RNA.

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