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Ricin Antibodies’ Neutralizing Capacity against Different Ricin Isoforms and Cultivars

Authors
  • Orsini Delgado, Maria Lucia1
  • Avril, Arnaud2
  • Prigent, Julie1
  • Dano, Julie1
  • Rouaix, Audrey1
  • Worbs, Sylvia3
  • Dorner, Brigitte G.3
  • Rougeaux, Clémence2
  • Becher, François1
  • Fenaille, François1
  • Livet, Sandrine1
  • Volland, Hervé1
  • Tournier, Jean-Nicolas2
  • Simon, Stéphanie1
  • 1 (H.V.)
  • 2 (J.-N.T.)
  • 3 (B.G.D.)
Type
Published Article
Journal
Toxins
Publisher
MDPI AG
Publication Date
Jan 29, 2021
Volume
13
Issue
2
Identifiers
DOI: 10.3390/toxins13020100
PMID: 33573016
PMCID: PMC7911099
Source
PubMed Central
Keywords
License
Green

Abstract

Ricin, a highly toxic protein from Ricinus communis , is considered a potential biowarfare agent. Despite the many data available, no specific treatment has yet been approved. Due to their ability to provide immediate protection, antibodies (Abs) are an approach of choice. However, their high specificity might compromise their capacity to protect against the different ricin isoforms (D and E) found in the different cultivars. In previous work, we have shown the neutralizing potential of different Abs (43RCA-G1 (anti ricin A-chain) and RB34 and RB37 (anti ricin B-chain)) against ricin D. In this study, we evaluated their protective capacity against both ricin isoforms. We show that: (i) RB34 and RB37 recognize exclusively ricin D, whereas 43RCA-G1 recognizes both isoforms, (ii) their neutralizing capacity in vitro varies depending on the cultivar, and (iii) there is a synergistic effect when combining RB34 and 43RCA-G1. This effect is also demonstrated in vivo in a mouse model of intranasal intoxication with ricin D/E (1:1), where approximately 60% and 40% of mice treated 0 and 6 h after intoxication, respectively, are protected. Our results highlight the importance of evaluating the effectiveness of the Abs against different ricin isoforms to identify the treatment with the broadest spectrum neutralizing effect.

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