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Ribosomal protein L24 defect in belly spot and tail (Bst), a mouse Minute.

Authors
  • Oliver, Edward R
  • Saunders, Thomas L
  • Tarlé, Susan A
  • Glaser, Tom
Type
Published Article
Journal
Development (Cambridge, England)
Publication Date
Aug 01, 2004
Volume
131
Issue
16
Pages
3907–3920
Identifiers
PMID: 15289434
Source
Medline
License
Unknown

Abstract

Ribosomal protein mutations, termed Minutes, have been instrumental in studying the coordination of cell and tissue growth in Drosophila. Although abundant in flies, equivalent defects in mammals are relatively unknown. Belly spot and tail (Bst) is a semidominant mouse mutation that disrupts pigmentation, somitogenesis and retinal cell fate determination. Here, we identify Bst as a deletion within the Rpl24 riboprotein gene. Bst significantly impairs Rpl24 splicing and ribosome biogenesis. Bst/+ cells have decreased rates of protein synthesis and proliferation, and are outcompeted by wild-type cells in C57BLKS<-->ROSA26 chimeras. Bacterial artificial chromosome (BAC) and cDNA transgenes correct the mutant phenotypes. Our findings establish Bst as a mouse Minute and provide the first detailed characterization of a mammalian ribosomal protein mutation.

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