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Rho-associated protein kinase inhibitor induced morphological changes in type VI collagen in the human trabecular meshwork.

Authors
  • Okamoto, Mizu1
  • Nagahara, Miyuki2
  • Tajiri, Takuma3
  • Nakamura, Naoya4
  • Fukunishi, Nahoko5
  • Nagahara, Kentaro6
  • 1 Ophthalmology, Tokai University Hachioji Hospital, Hachioji, Japan. , (Japan)
  • 2 Ophthalmology, National Center for Global Health and Medicine Hospital, Shinjuku-ku, Japan [email protected] , (Japan)
  • 3 Diagnostic Pathology, Tokai University Hachioji Hospital, Hachioji, Japan. , (Japan)
  • 4 Diagnostic Pathology, Tokai University School of Health Sciences, Isehara, Japan. , (Japan)
  • 5 Support Center for Medical Research and Education, Tokai University School of Health Sciences, Isehara, Japan. , (Japan)
  • 6 Mathematics, Tokyo Institute of Technology-Ookayama Campus, Meguro-ku, Japan. , (Japan)
Type
Published Article
Journal
British Journal of Ophthalmology
Publisher
BMJ
Publication Date
Mar 01, 2020
Volume
104
Issue
3
Pages
392–397
Identifiers
DOI: 10.1136/bjophthalmol-2018-312991
PMID: 31201167
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

To investigate morphological changes in type VI collagen in the human trabecular meshwork associated with the rho kinase inhibitor ripasudil. This cross-sectional study evaluated the effects of ripasudil eye drop administration (RA) or no ripasudil eye drop administration (NRA) in patients with primary open-angle glaucoma (POAG; age range 60-80 years) who underwent conventional outflow reconstruction between December 2015 and September 2016 at Tokai University Hachioji Hospital. The juxtacanalicular tissue was removed and imaged using transmission electron microscopy. Type VI collagen comprises cross-banded aggregates with transverse bands 30 nm apart repeating every 105 nm. The transverse bands are called the outer rod-like region (ORR) and the intervals are called the inner rod-like region (IRR). The waveform intensity in the type VI collagen was analysed in electron micrographs using Fourier transformation to detect the IRR and ORR borders. Ten eyes of 10 patients were included (n=5/group). The baseline characteristics did not differ significantly between groups. ORR width was significantly smaller in the RA group (37.85±3.43 nm) than in the NRA group (50.62±5.23 nm, p<0.05), whereas IRR width was significantly greater in the RA group (70.68±10.84 nm) than in the NRA group (58.19±5.34 nm, p<0.05). Morphological changes in the type VI collagen total width tended to correlate with the duration of ripasudil administration (r=0.9, p=0.08). Ripasudil administration in patients with POAG induced morphological changes in type VI collagen. Patients with POAG administered RA had a significantly smaller ORR width and a significantly greater IRR width than patients with POAG not administered RA. © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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