AbstractFeeding of the escalating population has subjected today’s agriculture to massive farming that has shattered soil structure, organic carbon and fertility. The vulnerability of applied organics to high temperature make them inappropriate under such circumstances. The help of indigenous exopolysaccharides (EPS) secreting microflora (Pre-isolated (M2, M3, M11, M16, M19 and M22)) was taken in an incubation experiment for the restoration of soil structure and organic carbon. M22 yielded 44.5% aggregation, 8.57 and 6 g kg–1 total and macroaggregate carbon, 220 and 311.2 mg kg–1 dissolved and microbial biomass carbon and 13.143 mg k–1 CO2 evolved in soils having moisture at 100% available water contents (AWC). At the end of the experiment M2, M19 and M22 yielded 43.87, 42.58 and 42.82% (0.25 to >1 mm) water-stable aggregates (WSA) that also carried 5.31, 5.75 and 5.41 g kg–1 carbon, respectively. Microbial activity as CO2 evolved (13.13, 13.73 and 14.11), solution (217.37, 225. 98 and 218.61), biomass carbon (300.93, 287.6 and 303.51) mg kg–1 and total organic carbon (TOC) (8.52, 8.67 and 8.73) g kg–1 were found highest in M2, M19 and M22 treated soils, respectively. Saccharide glues excreted from microbiota, flocculate soil particles to formulate aggregates, entrapping carbon for structural stabilization and organics restoration.