Middle ear effusions represent respiratory secretions which are physiologically important to functional mucocilliary transport systems. Knowledge of middle ear mucus and methods of facilitating clearance continue to be important to cleft palate patients. Middle ear mucus was collected from the ears of children with cleft palate before surgical correction. Samples were dialyzed and lyophilized to yield mucus powder. This mucus was reconstituted at various nondialyzable solids (NDS) concentrations in TRIS-CI buffer. A physiochemical study of the middle ear mucus was then undertaken, since a mucociliary transport defect leading to serous otitis media (SOM) is an etiologic possibility.