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A review of traditional and contemporary assays for direct and indirect detection of Equid herpesvirus 1 in clinical samples.

Authors
  • Balasuriya, Udeni B R1
  • Crossley, Beate M2
  • Timoney, Peter J2
  • 1 Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY (Balasuriya, Timoney)California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA (Crossley) [email protected]
  • 2 Maxwell H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY (Balasuriya, Timoney)California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California, Davis, CA (Crossley).
Type
Published Article
Journal
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Publication Date
Nov 01, 2015
Volume
27
Issue
6
Pages
673–687
Identifiers
DOI: 10.1177/1040638715605558
PMID: 26472746
Source
Medline
Keywords
License
Unknown

Abstract

Equid herpesvirus 1 (EHV-1) is one of the most economically important equine viral pathogens. Its clinical manifestations in horses vary from acute upper respiratory tract disease, abortion, or neonatal death, to neurological disease termed equine herpesviral myeloencephalopathy, which may lead to paralysis and a fatal outcome. Successful identification of EHV-1 infection in horses depends on a variety of factors such as suitable case selection with emphasis on timing of sample collection, selection of appropriate sample(s) based on the clinical manifestations, application of relevant diagnostic technique(s) and/or test(s), and careful evaluation and interpretation of laboratory results. Several traditional serologic and virus isolation assays have been described; however, these assays have inherent limitations that prevent rapid and reliable detection of EHV-1. The advent of molecular biologic techniques has revolutionized the diagnosis of infectious diseases in humans and animal species. Specifically, polymerase chain reaction (PCR)-based assays have allowed detection of nucleic acid in clinical specimens precisely and rapidly as compared to the traditional methods that detect the agent or antigen, or agent-specific antibodies in serum. The new molecular methods, especially real-time PCR, can be a very useful means of EHV-1 detection and identification. Veterinarians involved in equine practice must be aware of the advantages and disadvantages of various real-time PCR assays, interpretation of viral genetic marker(s), and latency in order to provide the best standard of care for their equine patients.

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