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Reversible AChE inhibitors in C. elegans vs. rats, mice.

Authors
  • Melstrom, Paul C
  • Williams, Phillip L
Type
Published Article
Journal
Biochemical and biophysical research communications
Publication Date
May 25, 2007
Volume
357
Issue
1
Pages
200–205
Identifiers
PMID: 17418813
Source
Medline
License
Unknown

Abstract

We are investigating whether Caenorhabditis elegans could be used as a screen for vertebrates by comparing the responses of components of its cholinergic system to well-characterized toxicants. We assessed whether C. elegans displays similar toxicity as rats and mice to reversible acetylcholinesterase (AChE) inhibitors, and sought to corroborate that the toxicity mechanism is the same. To determine relative potencies, movement-concentration curves were generated, 50th percentiles for movement were located, ranked and compared statistically to rat and mouse oral acute LD50s. The ranking was significantly correlated to rat and mouse rankings (alpha=0.05). We measured a concentration-dependent decrease in AChE activity correlating to a decrease in movement for each carbamate, suggesting that the mechanism of toxicity is the same. Finally, as seen in mammals, inhibition of AChE activity occurred before a movement decrease. The response of C. elegans to carbamate exposure shows significant correlation to rat and mouse data.

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