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Reverse transcriptase-PCR assay for detection of hog cholera virus.

Authors
  • M Harding
  • C Lutze-Wallace
  • I Prud'Homme
  • X Zhong
  • J Rola
Publication Date
Oct 01, 1994
Source
PMC
Keywords
License
Unknown

Abstract

A reverse transcriptase-PCR strategy was developed for the detection of hog cholera virus. Hog cholera virus template was amplified from tissue culture fluids and from tissues and blood of infected pigs, but not from samples containing other pestiviruses. Restriction endonuclease analysis identified samples as historic or recent isolates.

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