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Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer

Authors
  • Wilson, Marcus D.1, 2
  • Renault, Ludovic1, 3
  • Maskell, Daniel P.4, 5
  • Ghoneim, Mohamed6, 7
  • Pye, Valerie E.4
  • Nans, Andrea8
  • Rueda, David S.6, 7
  • Cherepanov, Peter4, 9
  • Costa, Alessandro1
  • 1 The Francis Crick Institute, Macromolecular Machines Laboratory, London, NW1 1AT, UK , London (United Kingdom)
  • 2 University of Edinburgh, Wellcome Centre for Cell Biology, Edinburgh, EH9 3JR, UK , Edinburgh (United Kingdom)
  • 3 University of Leiden, NeCEN, Leiden, 2333CC, Netherlands , Leiden (Netherlands)
  • 4 The Francis Crick Institute, Chromatin structure and mobile DNA Laboratory, London, NW1 1AT, UK , London (United Kingdom)
  • 5 Faculty of Biological Sciences, Leeds, LS2 9JT, UK , Leeds (United Kingdom)
  • 6 MRC London Institute for Medical Science, Single Molecule Imaging Group, London, W12 0NN, UK , London (United Kingdom)
  • 7 Imperial College London, Molecular Virology, Department of Medicine, London, W12 0NN, UK , London (United Kingdom)
  • 8 The Francis Crick Institute, Structural Biology Science Technology Platform, London, NW1 1AT, UK , London (United Kingdom)
  • 9 St-Maryʹs Campus, Norfolk Place, Department of Medicine, Imperial College London, London, W2 1PG, UK , London (United Kingdom)
Type
Published Article
Journal
Nature Communications
Publisher
Springer Nature
Publication Date
Sep 13, 2019
Volume
10
Issue
1
Identifiers
DOI: 10.1038/s41467-019-12007-w
Source
Springer Nature
License
Green

Abstract

Retroviral integrases catalyze the insertion of viral DNA into the host cell DNA and can use nucleosomes as substrates for integration. Here the authors present the 3.9 Å cryo-EM structure of prototype foamy virus integrase after strand transfer into nucleosomal DNA, which together with single-molecule FRET measurements provides evidence for a DNA looping and sliding mechanism of integrases.

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