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Response and tolerance of toxigenic Vibro cholerae O1 to cold temperatures

Authors
  • Carroll, Jeffrey W.1
  • Mateescu, Madalina C.1
  • Chava, Kiran1
  • Colwell, Rita R.2, 3
  • Bej, Asim K.1
  • 1 University of Alabama at Birmingham, Department of Biology, 1300 University Boulevard, Birmingham, AL, 35294-1170, USA , Birmingham
  • 2 University of Maryland, Department of Cell and Molecular Biology, College Park, MD, 20742, USA
  • 3 University of Maryland Biotechnology Institute, Center for Marine Biotechnology, Pratt Street, Baltimore, MD, 21202-3101, USA , Baltimore
Type
Published Article
Journal
Antonie van Leeuwenhoek
Publisher
Springer-Verlag
Publication Date
Sep 01, 2001
Volume
79
Issue
3-4
Pages
377–384
Identifiers
DOI: 10.1023/A:1012004725373
Source
Springer Nature
Keywords
License
Yellow

Abstract

Survival and tolerance at cold temperatures, the differentially expressed cellular proteins, and cholera toxin (CTX) production were evaluated in Vibrio cholerae O1. Rapid loss of culturability and change to distinct coccoid morphology occurred when cultures of V. cholerae O1 were exposed to 5°C directly from 35°C. Also, cultures of V. cholerae first exposed to 15°C for 2 h and then maintained at 5°C failed to exhibit an adaptive response, instead a rapid loss of viable plate count was noticed. Results from Western blot experiments revealed the absence of a major cold shock protein, CS7.4. Also, a decreased level of CTX was noticed in V. cholerae O1 cultures exposed to 5 or 15°C after first being exposed to 15°C for 2 h, followed by transfer to 5°C. Reduced expression of CTX at cold temperatures, compared to the cultures maintained at 35°C, may be a result of decreased cellular metabolic activity. When V. cholerae O1 cultures were exposed to 15°C for 2 h, elevated expressions of 8, 26 and 194 kDa, and decreased expression of 28 and 183 kDa proteins occurred. It is suggested that these differentially expressed cold-responsive proteins are involved in regulating culturability and conversion to a coccoid cell morphology in V. cholerae O1.

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