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Respective stemness and chondrogenic potential of mesenchymal stem cells isolated from human bone marrow, synovial membrane, and synovial fluid

Authors
  • Neybecker, Paul1
  • Henrionnet, Christel1
  • Pape, Elise1, 2
  • Grossin, Laurent1
  • Mainard, Didier1, 3
  • Galois, Laurent1, 3
  • Loeuille, Damien1, 4
  • Gillet, Pierre1, 2
  • Pinzano, Astrid1, 4, 5
  • 1 Biopôle de l’Université de Lorraine, Campus Brabois-Santé, 9 Avenue de la Forêt de Haye, BP 20199, Vandœuvre-Lès-Nancy, F54505, France , Vandœuvre-Lès-Nancy (France)
  • 2 CHRU de Nancy-Brabois, 5 Rue du Morvan, Vandœuvre-lès-Nancy, F54511, France , Vandœuvre-lès-Nancy (France)
  • 3 CHRU Nancy, 29 avenue du Maréchal de Lattre de Tassigny CO 60034, Nancy, F54035, France , Nancy (France)
  • 4 Bâtiment des Spécialités Médicales, 5 rue du Morvan, Vandœuvre-lès-Nancy, F54511, France , Vandœuvre-lès-Nancy (France)
  • 5 Hôpital de Brabois, Bâtiment Spécialités Médicales, Vandœuvre lès Nancy, F54511, France , Vandœuvre lès Nancy (France)
Type
Published Article
Journal
Stem Cell Research & Therapy
Publisher
Springer Nature America, Inc
Publication Date
Jul 25, 2020
Volume
11
Issue
1
Identifiers
DOI: 10.1186/s13287-020-01786-5
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundMSCs isolated from bone marrow (BM-MSCs) have well-established chondrogenic potential, but MSCs derived from the synovial membrane (SM-MSCs) and synovial fluid (SF-MSCs) are thought to possess superior chondrogenicity. This study aimed to compare the in vitro immunophenotype and trilineage and chondrogenic potential of BM-MSCs to SM-MSCs and SF-MSCs.MethodsMSCs were isolated from bone marrow (BM-MSCs), synovial membrane (SM-MSCs), and synovial fluid (SF-MSCs) extracted from the hips (BM) and knees (SM and SF) of advanced OA patients undergoing arthroplasty. Flow cytometric analysis was used at P2 to evaluate cell stemness. The trilinear differentiation test was performed at P2. At P3, MSC-seeded collagen sponges were cultured in chondrogenic medium for 28 days. Chondrogenic gene expression was quantified by qRT-PCR. Finally, the implants were stained to assess the deposition of proteoglycans and type II collagen.ResultsDespite variability, the immunophenotyping of BM-MSCs, SM-MSCs, and SF-MSCs was quite similar. All cell types were positive for the expression of stem cell markers and negative for exclusion markers. Additionally, chondrogenic differentiation and hypertrophy were more pronounced in BM-MSCs (ACAN, SOX9, COL2B, and COL10A) than in SF-MSCs, with SM-MSCs having intermediate characteristics. Concerning matrix synthesis, the three cell types were equipotent in terms of GAG content, while BM-MSC ECM synthesis of type II collagen was superior.ConclusionsChondrogenic MSCs are easily collected from SM and SF in advanced human OA, but in vitro chondrogenesis that is superior to age-matched BM-MSCs should not be expected. However, due to intra-articular priming, SF-MSCs did not overexpress hypertrophic gene.

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