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Resistance and Raman spectroscopy analysis of Parageobacillus thermantarcticus spores after γ-ray exposure

Authors
  • Romano, Ida1
  • De Angelis, Annalisa2
  • Poli, Annarita1
  • Ragni, Pietro3
  • Lilla, Laura3
  • Zito, Gianluigi2
  • Nicolaus, Barbara1
  • De Luca, Anna Chiara2
  • Di Donato, Paola1, 4
  • 1 National Research Council of Italy, Institute of Biomolecular Chemistry, Via Campi Flegrei 34, Pozzuoli, Naples, 80078, Italy , Pozzuoli, Naples (Italy)
  • 2 National Research Council of Italy, Institute of Protein Biochemistry, Via P. Castellino 111, Naples, 80131, Italy , Naples (Italy)
  • 3 Research Area of Rome, National Council Research of Italy, Institute of Chemical Methodologies, Via Salaria km 29.300, Monterotondo S., Rome, 00015, Italy , Monterotondo S. (Italy)
  • 4 University of Naples “Parthenope”, Centro Direzionale Isola C4, Department of Science and Technology, Naples, 80143, Italy , Naples (Italy)
Type
Published Article
Journal
Extremophiles
Publisher
Springer-Verlag
Publication Date
Aug 17, 2018
Volume
22
Issue
6
Pages
931–941
Identifiers
DOI: 10.1007/s00792-018-1049-0
Source
Springer Nature
Keywords
License
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Abstract

Spores of the genus Bacillus are able to resist ionizing radiations and therefore they are a suitable biological model for studies in Astrobiology, i.e. the multidisciplinary approach to the study of the origin and evolution of life on Earth and in the universe. The resistance to γ-radiation is an important issue in Astrobiology in relation to the search for bacterial species that could adapt to life in space. This study investigates the resistance of spores of the thermophilic bacteria Parageobacillus thermantarcticus to γ-rays. The analysis of spores’ response to irradiation at a molecular level is performed by means of Raman spectroscopy that allows to get insights in the sequence of events taking place during inactivation. The role of the γ-rays’ dose in the inactivation of spores is also investigated, allowing to highlight the mechanism(s) of inactivation including DNA damage, protein denaturation and calcium dipicolinate levels.

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