Recently, a new type of CD8+ T-cell subset, namely, the chemokine (C-X-C motif) receptor 5 (CXCR5+) cluster of differentiation (CD8+) T-cell subset (also called the follicular cytotoxic T-cell (TFC) subgroup), has been discovered around B-cell follicles. The discovery has aroused widespread interest. However, the processes and mechanisms of TFCs taking part in the immune response of the germinal center and their specific roles must still be clearly identified. This article reviews domestic and foreign studies on factors regulating the phenotype, physiological functions, maturity, and differentiation of TFCs and roles and clinical significance of these cells in HIV infection. This review has shown good application prospects for TFCs. The author believes that further studies on TFCs can provide another tool for cytotherapy to control or cure chronic viral infections or tumors.